PLoS Biology November 25, 2025

A framework to determine active neurons and networks within the mouse brain reveals how brain activity changes over the course of the day

by Guanhua Sun, Tomoyuki Mano, Shoi Shi, Alvin Li, Koji L Ode, Alex Rosi-Andersen, Erica Pedron, Steven A. Brown, Hiroki R Ueda, Konstantinos Kompotis, Daniel B. Forger The mouse brain’s activity changes drastically over a day despite being generated from the same neurons and physical connectivity. To better understand this, we develop an experimental-computational pipeline to determine which neurons and networks are most active at different times of the day. We genetically mark active neurons