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Morphogenesis and morphometry of brain folding patterns across species
School of Engineering and Applied Sciences, Harvard University, United States
Department of Mathematics, The Chinese University of Hong Kong, Hong Kong
Institut Pasteur, Université Paris Cité, Unité de Neuroanatomie Appliquée et Théorique, France
Department of Physics, Harvard University, United States
Department of Organismic and Evolutionary Biology, Harvard University, United States
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Biophysical basis for brain folding and misfolding patterns in ferrets and humans
Morphogenesis and morphometry of brain folding patterns across species
study presents a cross-species and cross-disciplinary analysis of cortical folding. The authors use a combination of physical gel models, computational simulations, and morphometric analysis, extending prior work in human brain development to macaques and ferrets. The findings support the hypothesis that mechanical forces driven by differential growth can account for major aspects of gyrification. The evidence presented is overall strong and
supports the central claims; the findings will be of broad interest in developmental neuroscience.
https://doi.org/10.7554/eLife.107138.3.sa0
: Findings that have theoretical or practical implications beyond a single subfield
: Appropriate and validated methodology in line with current state-of-the-art
During the peer-review process the editor and reviewers write an eLife Assessment that summarises the significance of the findings reported in the article (on a scale ranging from landmark to useful) and the strength of the evidence (on a scale ranging from exceptional to inadequate).
Evolutionary adaptations associated with the formation of a folded cortex in many mammalian brains are thought to be a critical specialization associated with higher cognitive function. The dramatic surface expansion and highly convoluted folding of the cortex during early development is a theme with variations that suggest the need for a comparative study of cortical gyrification. Here, we use a combination of physical experiments using gels, computational morphogenesis, and geometric morphometrics to study the folding of brains across different species. Starting with magnetic resonance images of brains of a newborn ferret, a fetal macaque, and a fetal human, we construct two-layer physical gel brain models that swell superficially in a solvent, leading to folding patterns similar to those seen in vivo. We then adopt a three-dimensional continuum model based on differential growth to simulate cortical folding in silico. Finally, we deploy a comparative morphometric analysis of the in vivo, in vitro, and in silico surface buckling patterns across species. Our study shows that a simple mechanical instability driven by differential growth suffices to explain cortical folding and suggests that variations in the tangential growth and different initial geometries are sufficient to explain the differences in cortical folding across species.
The most recognizable feature of the human brain is its surface folding patterns. In humans and many other mammals, the outer layer of the brain – the cerebral cortex – develops a complex pattern of ridges and grooves. These folds allow a large cortical surface to fit inside the skull and are closely linked to brain function.
Cortical folding can begin before birth, but it continues after birth as the gray matter cortex grows faster than the softer tissue beneath it, known as white matter. When a growing surface is constrained in this way, mechanical stresses build up and cause it to buckle and form sharp creases or sulci like those in the palm of one’s hand. Although genes control how brain cells grow and move, physical forces determine how this growth is translated into shape through mechanical instabilities. Different species, such as ferrets, macaques and humans, show distinct folding patterns, raising the question of whether these differences require specific biological mechanisms or can arise from a shared physical process.
Yin et al. asked whether brain folding across different mammalian species can be explained by the same basic physical mechanism: differential growth of the cortex relative to the underlying tissue. While a related study (Choi et al.) showed that this mechanism can reproduce folding in the human brain, it was unclear whether it could also account for the diversity of folding patterns seen across species. Resolving this question helps clarify how universal physical principles interact with biological growth during brain development.
The results show that a single mechanical mechanism can explain brain folding in ferrets, rhesus macaques and humans. Yin et al. combined physical experiments using soft gel models shaped like fetal brains that swell and fold when immersed in a solvent, computer simulations of growing brain tissue, and quantitative comparisons of folding patterns using mathematical frameworks. In all species studied, faster growth of the cortical layer caused the surface to buckle and form realistic folds. Differences between species were explained by variations in initial brain shape, and cortical growth rate, rather than by different folding rules. The close match between real brains, gel models, and simulations supports differential growth as a unifying explanation for cortical folding.
The work of Yin et al. provides a simple physical framework for understanding how brain folding arises across species. This suggests that genetic control of brain shape and cortical expansion could, over time, lead to different patterns of brain folding across species, as well as disorders associated with abnormal folding. However, before these insights can inform medical or evolutionary applications, future studies will need to link specific genes and cellular processes to the growth parameters used in the models and test whether the framework can explain individual differences and disease-related folding patterns, the subject of a companion paper in the same journal.
Although not all brains are folded, in many mammals, the folded cerebral cortex is known to be critically important for brain cognitive performance and highly dependent on the hierarchical structure of its morphology, cytoarchitecture, and connectivity (
). Brain function is thus related both to the topological structure of neural networks (
), as well as the geometry and morphology of the convoluted cortex (
), both of which serve to enable and constrain neuronal dynamics (
). Across species, cortical morphologies show a large diversity, as shown in
). And within our own species and in model organisms, such as the ferret used to study the genetic precursors of misfolding, cortical folding, and misfolding are known to be markers of healthy and pathological neurodevelopment, disease, and aging (
). Thus, a comparative study of cortical folding is essential for understanding brain morphogenesis and functionalization across evolution (
), during development as well as in pathological situations associated with disease.
The diversity of the cortical morphologies and developmental processes across species.
) Phylogenetic relationship of species. Adapted from
. Typical real brain surfaces of ferrets and primates are presented. Color represents mean curvature. Scale bars: 1 cm (estimated from
) Stained sections of mature brain tissue from ferret, rhesus macaque, and human. Scale bar: 10 mm. Adapted from
) 3D reconstruction of cortical surfaces of ferret, macaque, and human brains from fetal to adult. (
) Ferret: postnatal day 4, 10, 17, and adult maturation (
) Human: gestation day 175 (week 25), 210 (week 30), 231 (week 33), 273 (week 39), and adult maturation (
© 2009, Springer Nature. This figure1/Panel C,E was reprinted with permission fro